Standard Operating Procedure (SOP): Accumet AB 15 pH Meter

This video will demonstrate the standard operating
procedure for the Accumet AB15 pH meter. By the end of this video, you will be able to calibrate this machine and measure the pH of samples with it. The Accumet AB15 pH meter is connected to
an electrolyte-filled electrode. Turn on the pH meter by pressing STANDBY. Press SETUP twice to access the current calibration
memory, then press ENTER to clear the memory. Next, press SETUP until you find the pH buffer
group 2,4,7,10,12 and press ENTER to select this group. To turn the probe to ON position, turn the
purple ring on the probe until you can see an opening before use. This step is very important for accurate pH
measurement. Remove the pH probe from the storage bottle
carefully, rinse with dd water and gently dry with kimwipes. Calibrate the pH probe by first immersing
the pH probe in the pH 4 standard buffer. Be sure to secure the pH probe upright on
the stand. Press “Std” the access calibration mode,
then wait until the reading shows “stable” and you can press “Std” to enter the result. Please note that this meter does not have
the auto-read function. Rinse the pH probe with dd water and dry with kimwipes. You should always rinse the probe with dd water between solutions. Continue the calibration with standard buffers
pH 7.00 and pH 10.00. After calibration is complete, a slope will
show up very briefly. As long as it is within the range of 90% to
110%, it will indicate a good electrode. The pH meter is now ready for use. During the pH determination, you should make sure your sample is constantly stirred with a stir bar on a stir plate. Keep the pH probe upright on the stand and
lower into the sample. Be sure the pH probe never touches the stir
bar or the probe will be damaged. The pH meter will automatically begin reading
the sample. Record the pH when the value shows “stable”. When pH measurement is complete, rinse the pH probe with dd water, dry gently with kimwipes and store the probe in pH 4 buffer. Remember to turn the probe to OFF position
by turning the purple ring on the probe until you can no longer see an opening. This is very important because if the probe is left in the ON position, the electrolyte will evaporate over time and the probe will be damaged.

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