Waters Prep SFC 150 AP Introduction
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Welcome to the Waters Prep
SFC 150 AP introduction video. My name is Catharine Layton and I am
a Senior Application Chemist at Waters Corporation. Today I’m going to
give you an overview of the new, mass directed SFC purification
system, the Prep SFC 150 AP. The system is designed for high throughput
isolation of sample compounds in an automated fashion. It is widely employed in a variety of
industries such as medicinal chemistry, chemical materials,
natural products, and R&D. Let’s follow the typical
path for sample purification. The sample is injected from either a
modified stream injector or the Sample Manager, which provides flexible
formats and multiple open bed layouts. The sample bed options, are test
tubes, vials or microtiter plates. The updated P200x CO2 pump operates
in tandem with the Waters quaternary gradient module to deliver a total
combined flow rate of up to 150 mL/min. These flow rates are suitable for 10, 19, and 30 millimeter ID chiral and
achiral purification columns. Sample compounds are
detected by UV or UV-MS. Mass directed purification is driven
by QDa or SQD2 mass detectors, which now feature the Waters Isocratic
Solvent Manager (ISM) make-up pump. After detection, the sample moves through the updated
gas liquid separation chamber, also known as the GLS, to
remove CO2 from the cosolvent. The GLS design allows accurate fraction
isolation and minimizes fraction collection volumes. Decreased fraction collection volumes
reduce overall solvent evaporation time, and processing costs. Following the GLS, the purified sample is delivered back
into the open bed sample manager high capacity fraction collection system for
assortment into commonly used 18 and 25 millimeter i.d. tubes. Finally, the system is controlled by MassLynx /
FractionLynx operating on Windows 10. Thank you for listening.
For further information, please visit the Waters
website at waters.com/sfc150.

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